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It is an application for assessing genome assembly and annotation completeness. | It is an application for assessing genome assembly and annotation completeness. | ||
For more information see the [https://gitlab.com/ezlab/busco/blob/master/BUSCO_v3_userguide.pdf user manual]. | For more information see the [https://gitlab.com/ezlab/busco/blob/master/BUSCO_v3_userguide.pdf user manual]. | ||
== Available versions == | |||
The version 3.0.2 of BUSCO is installed as a module on cvmfs and accessible on all clusters. See below how to use it. For the [https://gitlab.com/ezlab/busco newer versions], it is possible to install them locally using a [[Python#Creating_and_using_a_virtual_environment|virtual environment]] as follow: | |||
{{Commands| | |||
~ $ module load python/3.7.4 | |||
~ $ git clone https://gitlab.com/ezlab/busco.git | |||
~ $ virtualenv /home/$USER/busco_env | |||
~ $ source /home/$USER/busco_env/bin/activate | |||
(busco_env) [~]$ pip install Biopython | |||
(busco_env) [~]$ cd ~/busco | |||
(busco_env) [~]$ python setup.py install | |||
(busco_env) [~]$ cp -r scripts test_data /home/$USER/busco_env/ | |||
}} | |||
and add "home/$USER/busco_env/scripts" to your path. | |||
== Usage == | == Usage == |