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However, GenomeAnalysisTK.jar must be in PATH. On our systems, the environmental variables <code>$EBROOTPICARD</code> for Picard (included in GATK >= 4) and <code>$EBROOTGATK</code> for GATK contain the path to the jar file, so the appropriate way to call GATK <= 3 is | However, GenomeAnalysisTK.jar must be in PATH. On our systems, the environmental variables <code>$EBROOTPICARD</code> for Picard (included in GATK >= 4) and <code>$EBROOTGATK</code> for GATK contain the path to the jar file, so the appropriate way to call GATK <= 3 is | ||
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===Multicore usage === <!--T:19--> | ===Multicore usage === <!--T:19--> | ||
Most GATK (>=4) tools are not multicore by default. This means that you should request only one core when calling | Most GATK (>=4) tools are not multicore by default. This means that you should request only one core when calling them. Some tools use threads in some of the computations (e.g. <code>Mutect2</code> has the <code>--native-pair-hmm-threads</code>) and therefore you can require more cpus (most of them with up to 4 threads) for these computations. GATK4, however, does provides <b>some</b> [https://gatk.broadinstitute.org/hc/en-us/articles/360035890591-Spark SPARK commands]: | ||
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